The Lung Microbiome Edited by Michael J. Cox, Markus J. Ege and Erika von Mutius Pantone 200 CMJN (darker) Cyan 0 Magenta 100 Yellow 70 Black 14 Pantone 647 CMJN Cyan 100 Magenta 56 Yellow 0 Black 24 Pantone PASTEL 9081 CMJN Cyan 0 Magenta 0 Yellow 6 Black 8 ERS monograph ERS monograph Print ISSN: 2312-508X Online ISSN: 2312-5098 Print ISBN: 978-1-84984-101-6 Online ISBN: 978-1-84984-102-3 March 2019 €60.00 Pantone 200 CMJN (darker) Cyan 0 Magenta 100 Yellow 70 Black 14 Pantone 647 CMJN Cyan 100 Magenta 56 Yellow 0 Black 24 Pantone PASTEL 9081 CMJN Cyan 0 Magenta 0 Yellow 6 Black 8 Studying the lung microbiome requires a specialist approach to sampling, laboratory techniques and statistical analysis. This Monograph introduces the techniques used and discusses how respiratory sampling, 16S rRNA gene sequencing, metagenomics and the application of ecological theory can be used to examine the respiratory microbiome. It examines the different components of the respiratory microbiome: viruses and fungi, in addition to the more frequently studied bacteria. It also considers a range of contexts from the paediatric microbiome and how this develops to disease of all ages including asthma and chronic obstructive pulmonary disease, chronic suppurative lung diseases, interstitial lung diseases, acquired pneumonias, transplantation, cancer and HIV, and the interaction of the respiratory microbiome and the environment. The Lung Microbiome ERS monograph 83